Taking place before the events in Undercover: Operation Wintersun, Dual Motives allows players not only to control John Russell, but have access to a second playable character in Audrey, the secretary of the facility's director.

Both screens of the DS are used, when Russell and Audrey investigate. Working separately, the player is in control of one of them at a time, directing them via the touchscreen and stylus. The other screen displays the currently inactive character, which he can switch over to at any time.